450k infinium microarray dna methylation data (INFINIUM Inc)
90
Structured Review
INFINIUM Inc
450k infinium microarray dna methylation data
10 ). Data were analyzed for statistical significance using an ordinary one‐way ANOVA (* p < 0.05, ** p < 0.01). H1‐0 levels across two leukemia patient cohorts derived from the (F) PeCan St. Jude database 30 , 31 and (G) an expression microarray dataset (accession number GSE87070 32 ). The number of patients per leukemia entity and mean expression is indicated. Data were analyzed for statistical significance using an ordinary one‐way ANOVA (*** p < 0.001). (H) H1‐0 expression was quantified by RT‐qPCR in PDX samples ( n = 9). Mean expression ± standard deviation is shown. (I) RNA‐seq expression levels of H1‐0 in control and ETV6 shRNA‐transduced REH cells. Data are derived from E‐MTAB‐10308 11 and are normalized to control shRNA. Mean expression ± standard deviation is indicated. Statistical significance was determined by performing a one‐way ANOWA (*** p < 0.001). (J) Pearson correlation of H1‐0 and RUNX1 expression in healthy bone marrow cells ( n = 71) derived from the MILE study (R2 platform, accession number GSE13159 33 ). " width="250" height="auto" />
450k Infinium Microarray Dna Methylation Data, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+microarray+data/infinium+humanmethylation450+beadchip/pmc11962653-118-5-6
Average 90 stars, based on 1 article reviews
10 ). Data were analyzed for statistical significance using an ordinary one‐way ANOVA (* p < 0.05, ** p < 0.01). H1‐0 levels across two leukemia patient cohorts derived from the (F) PeCan St. Jude database 450k Infinium Microarray Dna Methylation Data, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+microarray+data/infinium+humanmethylation450+beadchip/pmc11962653-118-5-6
Average 90 stars, based on 1 article reviews
450k infinium microarray dna methylation data - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "H1‐0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemia"
Article Title: H1‐0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemia
Journal: HemaSphere
doi: 10.1002/hem3.70116
10 ). Data were analyzed for statistical significance using an ordinary one‐way ANOVA (* p < 0.05, ** p < 0.01). H1‐0 levels across two leukemia patient cohorts derived from the (F) PeCan St. Jude database Figure Legend Snippet: H1‐0 is consistently upregulated in preleukemia and BCP‐ALL expressing ETV6::RUNX1 . (A) Principal component analysis (PCA) plot of ETV6::RUNX1 + (E::R) and wild‐type (WT) hiPSC transcriptome profiles based on all detected genes ( n = 16,328). (B) Hierarchical clustering analysis of differentially expressed genes (absolute fold change > 2 and p < 0.05) between ETV6::RUNX1 + and WT hiPSCs detected by RNA‐seq. (C) H1‐0 expression levels determined by RT‐qPCR in ETV6::RUNX1 + and WT hiPSCs subjected to RNA‐seq. Values were normalized to HW8 WT expression levels as well as to GAPDH expression. (D) Representative Western blot analysis of ETV6::RUNX1, H1‐0, ETV6, and β‐actin levels in ETV6::RUNX1 + and WT hiPSCs. (E) H1‐0 levels in HSCs (CD19‐CD34+CD45RA‐), IL7R+ (CD19‐CD34+CD45RA+IL7R+), and pro‐B (CD19+CD34+) cells differentiated from ETV6::RUNX1 + or reverted MIFF3 hiPSCs, and fetal liver cells. Data are derived from an RNA‐seq dataset by Böiers et al. (accession number E‐MTAB‐6382
Techniques Used: Expressing, RNA Sequencing, Quantitative RT-PCR, Western Blot, Derivative Assay, Microarray, Standard Deviation, Control, shRNA
41 ). Expression is shown for microarray probe 208886_at. Each dot represents a single patient. (D) H1‐0 DNA methylation in different leukemia entities is visualized as a heatmap with each column corresponding to a single patient (accession number GSE49032 Figure Legend Snippet: ETV6::RUNX1 induces H1‐0 promoter activation . (A) Schematic representation of the H1‐0 locus, including the 512‐bp region (nucleotides −351 to +161 from TSS) encompassing promoter‐like signature EH38E2163184 (ENCODE). The H1‐0 CpG island (CGI) shore and 450K Infinium array probes are indicated. (B) 293T cells were transfected with a vector encoding the H1‐0 promoter‐like signature indicated in (A) , together with the empty pcDNA3.1 vector or pcDNA3.1 expressing either ETV6::RUNX1 or RUNX1, and a vector expressing Renilla luciferase. Luciferase activities were normalized to Renilla luciferase activity and the empty vector control. Data represent mean values of three independent replicates ± standard deviation. Significance was calculated using an ordinary one‐way ANOVA (*** p < 0.001). Representative protein levels of ETV6::RUNX1, RUNX1, and β‐actin determined by Western blot are shown. (C) Pearson correlation of H1‐0 expression and mean DNA methylation of the H1‐0 CGI shore probes cg07141002 and cg01883777 in leukemia patients (accession number GSE49032
Techniques Used: Activation Assay, Transfection, Plasmid Preparation, Expressing, Luciferase, Activity Assay, Control, Standard Deviation, Western Blot, DNA Methylation Assay, Microarray
45 ). B cell precursor fractions are HSCs (CD34+CD19‐IgM‐), pro‐B cells (CD34+CD19+IgM‐), pre‐B cells (CD34‐CD19+IgM‐) and immature B cells (CD34‐CD19+IgM+). (B) H1‐0 expression in healthy B cell precursor stages derived from a published expression microarray dataset (accession number GSE24759 Figure Legend Snippet: H1‐0 expression decreases during hematopoiesis . (A) H1‐0 expression in ETV6::RUNX1 + BCP‐ALL ( n = 6) and healthy B cell precursor stages derived from a published RNA‐seq dataset (accession number GSE115656
Techniques Used: Expressing, Derivative Assay, RNA Sequencing, Microarray, Standard Deviation
Related Articles
DNA Methylation Assay:Article Title: Recent advances in Forensic DNA Phenotyping of appearance, ancestry and age. Article Snippet: .. The first analyses to select DNA methylation age predictors based on the Infinium Article Title: H1‐0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemia Article Snippet: .. Hence, we analyzed previously published Article Title: eClock : An ensemble-based method to accurately predict ages with a biased distribution from DNA methylation data Article Snippet: .. The third module is for Infinium Article Title: Epigenetic identification of ADCY4 as a biomarker for breast cancer: an integrated analysis of adenylate cyclases. Article Snippet: .. DNA methylation data for each probe were determined using Article Title: Liquid Biopsy in Alzheimer’s Disease Patients Reveals Epigenetic Changes in the PRLHR Gene Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient ( ). .. In this study, we used our Article Title: Liquid Biopsy in Alzheimer's Disease Patients Reveals Epigenetic Changes in the PRLHR Gene. Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient (Supplementary Figure S1). .. In this study, we used our previous DNA methylation microarray data obtained by the Infinium Microarray:Article Title: Recent advances in Forensic DNA Phenotyping of appearance, ancestry and age. Article Snippet: .. The first analyses to select DNA methylation age predictors based on the Infinium Article Title: H1‐0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemia Article Snippet: .. Hence, we analyzed previously published Article Title: eClock : An ensemble-based method to accurately predict ages with a biased distribution from DNA methylation data Article Snippet: .. The third module is for Infinium Article Title: Epigenetic identification of ADCY4 as a biomarker for breast cancer: an integrated analysis of adenylate cyclases. Article Snippet: .. DNA methylation data for each probe were determined using Article Title: Liquid Biopsy in Alzheimer’s Disease Patients Reveals Epigenetic Changes in the PRLHR Gene Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient ( ). .. In this study, we used our Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Article Title: Liquid Biopsy in Alzheimer's Disease Patients Reveals Epigenetic Changes in the PRLHR Gene. Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient (Supplementary Figure S1). .. In this study, we used our previous DNA methylation microarray data obtained by the Infinium RNA Sequencing:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Only unique primary (−01A) and metastatic (-06A) tumor samples, as well as unique normal adjacent tissues (−11A), for which both RNA-seq and Methylation:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Only unique primary (−01A) and metastatic (-06A) tumor samples, as well as unique normal adjacent tissues (−11A), for which both RNA-seq and Article Title: Liquid Biopsy in Alzheimer’s Disease Patients Reveals Epigenetic Changes in the PRLHR Gene Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient ( ). .. In this study, we used our Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Article Title: Liquid Biopsy in Alzheimer's Disease Patients Reveals Epigenetic Changes in the PRLHR Gene. Article Snippet: After performing immunostaining with anti β-amyloid and anti p-tau antibodies, the hippocampal sections were examined and representative images were analyzed with ImageJ software v1.52d to obtain an average quantitative measure for each section and patient (Supplementary Figure S1). .. In this study, we used our previous DNA methylation microarray data obtained by the Infinium Clone Assay:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Derivative Assay:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Transfection:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) shRNA:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Plasmid Preparation:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) Control:Article Title: Transcriptional overlap links DNA hypomethylation with DNA hypermethylation at adjacent promoters in cancer Article Snippet: .. Clones were derived from hTERT-immortalized human male fibroblasts transfected with an anti-DNMT1 shRNA producing vector (pSh- DNMT1 ). ( B ) cDNA microarray data were analyzed to determine relative mRNA levels of DMNT1 , MAGEA6 and ( CT- ) GABRA3 (the two transcript variants cannot be distinguished by microarray), in the control hTERT fibroblast cell line (Ctrl) and in the three DNMT1-depleted clones (D8, D10 and D18). ( C ) |
